BIOLOGICAL CONTROL: TETRASTICHUS CULTURE

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Tetrastichus howardi is parasitic non stinging wasp that are endoparasitoids of insect pupae. Tetrastichus is a genus of hymenopteran insects of the family Eulophidae.
  •  Tetrastichus is Pupal parasitoid, it works against Sugarcane Borers Ex- Top Borer, Shoot Borer, Stem Borer, Root Borer etc.


Points to remember before Starting tetrastichus culture-
  •   Maintain the temperature 24-26 ˚c throughout the year in culture room.
  •   Maintain your culture room free from any kind of contamination.
Material and apparatus required for  tetrastichus culture-




  • Pupa of Silkworm as host of tetrastichus
  • Plastic Containers & Plastic small boxes
  • Tetra packs/ Polythene packs
  • Ethenol
  • Cotton
  • Honey
  • Vitamin E tablets
Procedure-
  • Pick out the pupa from cocoons gently
  • Put one pupa in each plastic container
  • Put 15-20 tetrastichus adults in each box & capped it
  • Leave it for 24 hours for egg laying
  • Take out pupa from box after 24 hours & keep it in another box, leave it for 4-5 days without disturbing them for development of eggs. 
  • Put one another fresh pupa in previous box for egg laying.


Note-
  1. Life cycle of tetrastichus adult is just 6-7 days. when pupa is not available for multiplication at that time life of adult can be increased by 4-5 days giving them immunity bill as food material i.e. small cotton boll dipped in mixture of vitamin E tablets, honey, water & keep it in box.
  2. Generally in month of February to October cocoon is not easily available in Uttar Pradesh so to maintain culture throughout the year Immunity boll can be used to increase life of adults.


Packing & supply-
  • Packing of tetrastichus can be done in small polythene packs.
  • Each pack contain approx. 150-200 tetrastichus adults.
  • Supply of tetrastichus is to be done in ice box for maintaining the optimum temperature.


Cost & Dose/ha-
  • Cost of one tetra pack is Rs. 25
  • 8-10 packets 3 times 




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